2012Ganhan diqu nongye yanjiuRequires access

Effect of drought stress and rewater on protective enzyme activity and membrane lipid peroxidation in pea leaves during seedling stage

Juan Jiang

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Abstract

A pot experiment was conducted to investigate the effect of soil water on protective enzymes of superoxide dismutase(SOD),catalase(CAT) and peroxidase(POD) activity,and as well as on membrane lipid peroxidation including malonaldehyde (MDA) content and plasma membrane permeability(RC) in pea(Pisum sativum Linn.) leaves during seedling stage under drought stress and rewater conditions.The comprehensive evaluation on antioxidant capability of pea during seedling stage was also made.The results showed that drought stress declined SOD activity,and more remarkably with increasing stress intensity and time.Light drought stress for 10 d and serious drought stress all increased POD and CAT activity,and more obviously with increasing stress intensity and time.Rewater for 5 d produced partial compensation to CAT and SOD activity,and equal compensation to POD activity.Rewater for 10 d produced equal compensation to SOD activity and equal or over compensation to CAT and POD activity.Drought stress increased MAD content,and increased more with increasing stress intensity,and increased less with time.Rewater for 5 d and 10 d produced partial and equal compensation respectively to MDA content of pea leaves under drought stress for 5 d.Drought stress enhanced RC and more significantly with increasing stress intensity and time.Rewater for 5 d and 10 d produced partial and equal compensation respectively.

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What this paper is about

A pot experiment was conducted to investigate the effect of soil water on protective enzymes of superoxide dismutase(SOD),catalase(CAT) and peroxidase(POD) activity,and as well as on membrane lipid peroxidation including malonaldehyde (MDA) content and plasma membrane permeability(RC) in pea(Pisum sativum Linn.) leaves during seedling stage under drought stress and rewater conditions.The comprehensive evaluation on antioxidant capability of pea during seedling stage was also made.The results showed that drought stress declined SOD activity,and more remarkably with increasing stress intensity and time.Light drought stress for 10 d and serious drought stress all increased POD and CAT activity,and more obviously with increasing stress intensity and time.Rewater for 5 d produced partial compensation to CAT and SOD activity,and equal compensation to POD activity.Rewater for 10 d produced equal compensation to SOD activity and equal or over compensation to CAT and POD activity.Drought stress increased MAD content,and increased more with increasing stress intensity,and increased less with time.Rewater for 5 d and 10 d produced partial and equal compensation respectively to MDA content of pea leaves under drought stress for 5 d.Drought stress enhanced RC and more significantly with increasing stress intensity and time.Rewater for 5 d and 10 d produced partial and equal compensation respectively.

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Available abstract

A pot experiment was conducted to investigate the effect of soil water on protective enzymes of superoxide dismutase(SOD),catalase(CAT) and peroxidase(POD) activity,and as well as on membrane lipid peroxidation including malonaldehyde (MDA) content and plasma membrane permeability(RC) in pea(Pisum sativum Linn.) leaves during seedling stage under drought stress and rewater conditions.The comprehensive evaluation on antioxidant capability of pea during seedling stage was also made.The results showed that drought stress declined SOD activity,and more remarkably with increasing stress intensity and time.Light drought stress for 10 d and serious drought stress all increased POD and CAT activity,and more obviously with increasing stress intensity and time.Rewater for 5 d produced partial compensation to CAT and SOD activity,and equal compensation to POD activity.Rewater for 10 d produced equal compensation to SOD activity and equal or over compensation to CAT and POD activity.Drought stress increased MAD content,and increased more with increasing stress intensity,and increased less with time.Rewater for 5 d and 10 d produced partial and equal compensation respectively to MDA content of pea leaves under drought stress for 5 d.Drought stress enhanced RC and more significantly with increasing stress intensity and time.Rewater for 5 d and 10 d produced partial and equal compensation respectively.

Key concepts: Point of delivery, Seedling, Catalase, Superoxide dismutase, Pisum, Lipid peroxidation, Peroxidase, Enzyme assay

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Effect of drought stress and rewater on protective enzyme activity and membrane lipid peroxidation in pea leaves during seedling stage — Research Paper | ScholarLens