Expression of the Capsid Precursor Protein Gene of Foot-and-mouth Disease Virus and Green Fluorescent Protein Gene in BHK-21 Cells Mediated by Retroviral Vector

Here we constructed a retroviral vector mediated mammalian cell expression system of the capsid precursor protein of foot-and-mouth disease virus(FMDV).The recombinant retroviral vector pBABEpuro-P1-2A-EGFP was constructed by inserting capsid precursor protein gene(P1) of FMDV and enhance green fluorescent protein gene(EGFP) into pBABEpuro by turns.Both the recombinant retroviral vector and the pVSV-G plasmid were co-transfected into packaging cell GP2-293 by liposome-mediated transduction.And then,the pseudovirus were produced.The pseudovirus infected BHK-21 cells and resistance cells were screened by puromycin-resistant.The green fluorescent protein were observed under fluorescence microscope.The expression of capsid precursor protein gene of FMDV was detected by indirect immunofluorescence assay.The recombinant retroviral vector pBABEpuro-P1-2A-EGFP was constructed successfully.The capsid precursor protein of FMDV and green fluorescent protein could express in BHK-21 cells.The mammalian cell expression system of the capsid precursor protein of FMDV had been constructed successfully,which laid a foundation of development of FMDV subunit vaccine.