2009•Chinese Veterinary ScienceRequires access
Construction of a recombinant attenuated goatpox virus expressing the P1-2A and 3C genes of Asia 1 FMDV.
Zhang Qiang, Ju HouBin, Guohua Wu, Yan Xinmin, Li Jian, Zhu Haixia, Weimin Ma, Zheng Haixue, Zhu CaiZhu, Xuepeng Cai
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Abstract
In order to construct a recombinant attenuated goatpox virus(GTPV) expressing the P1-2A and 3C genes of Asia 1 foot-and-mouth disease virus(FMDV),the capsid protein precursor P1-2A and proteolytic ferment 3C gene of Asia 1 FMDV,enhanced green fluorescent protein(EGFP) gene and vaccinia virus promoter p7.5 were linked together to construct an eukaryotic gene expression cassette p7.5-EGFP-P12A3C.The cassette was cloned into pUC119-TK at the KpnⅠrestriction enzyme site to construct a goatpox virus transfer vector pTK-p7.5-EGFP-P12A3C.The transfer vector pTK-p7.5-EGFP-P12A3C was transfected into BHK-21 cells infected with GTPV AV 41 strain.Green fluorescence could be observed at hour 24 post-transfection.The result revealed that a recombinant goatpox virus expressing the interest proteins TK-/EGFP+/ FMDV P1-2A3C+/GTPV was successfully constructed.