Primary Culture of Osteoblasts of Neonatal Mice:a Comparative Research

Objective To confirm an alternative method for primary culture of osteoblasts through comparison of two primary culture methods. Methods Calvarias were dissected from newborn Kunming mice, osteoblasts were isolated with serum-containing collagenase digestion method and sequential digestion method respectively. The osteoblasts were observed under invert microscope, the growth curve was made with the application of MTT method, the rate of living osteoblasts was counted with trypan blue method respectively. Results Compared with the serum-containing collagenase digestion method, the sequential digestion method presented higher production of osteoblasts, higher cell survival rate (P0.01), little damage on osteoblasts and easier control of the digestion time. Conclusion The sequential digestion of calvaria method is simple, efficient and stable for primary osteoblasts culture.