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ESTABLISHMENT OF NEWBORN RAT OSTEOBLASTS CULTURE

Yongzhou Song

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Abstract

Objective To establish an experimental model of osteoblasts of newborn rat in vitro.Methods The skull of neonatal rats within 24 h was obtained and digested by compound collagenase digestion repeatedly.The cells were collected to culture in vitro.The osteoblasts were identified by cell morphology,and their biological characteristic was examined by HE,ALP,type I collagen and calvaria staining respectively.Results The cultured cells were osteoblasts and grew well.The cells were positive by ALP,type I collagen and calvaria staining cells.Conclusion The osteoblasts isolated by compound collagenase digestion have typical biological characteristic of osteoblasts.

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Objective To establish an experimental model of osteoblasts of newborn rat in vitro.Methods The skull of neonatal rats within 24 h was obtained and digested by compound collagenase digestion repeatedly.The cells were collected to culture in vitro.The osteoblasts were identified by cell morphology,and their biological characteristic was examined by HE,ALP,type I collagen and calvaria staining respectively.Results The cultured cells were osteoblasts and grew well.The cells were positive by ALP,type I collagen and calvaria staining cells.Conclusion The osteoblasts isolated by compound collagenase digestion have typical biological characteristic of osteoblasts.

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Available abstract

Objective To establish an experimental model of osteoblasts of newborn rat in vitro.Methods The skull of neonatal rats within 24 h was obtained and digested by compound collagenase digestion repeatedly.The cells were collected to culture in vitro.The osteoblasts were identified by cell morphology,and their biological characteristic was examined by HE,ALP,type I collagen and calvaria staining respectively.Results The cultured cells were osteoblasts and grew well.The cells were positive by ALP,type I collagen and calvaria staining cells.Conclusion The osteoblasts isolated by compound collagenase digestion have typical biological characteristic of osteoblasts.

Key concepts: Calvaria, Collagenase, In vitro, Osteoblast, Staining, Cell culture, Type I collagen, Chemistry

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