Novel Validated RP-HPLC Method for Simultaneous Estimation of Lisinopril and Amlodipine in Bulk and Tablet Dosage Form

A simple, reproducible and efficient reverse phase high performance liquid chromatographic method was developed for simultaneous determination of lisinopril(LSN) and amlodipine (AMD) in tablets. A column having 150 × 4.6 mm in isocratic mode with mobile phase containing acetonitrile: phosphate buffer (55:45; adjusted to pH 3.0) was used. The flow rate was 0.6 ml/min and effluent was monitored at 216 nm. The retention time 8 (min) and linearity range (μg/ml) for LSN and AMD were and (20-60,10-30), respectively. The developed method was found to be accurate, precise and selective for simultaneous determination of LSNand AMD in tablets.