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2012•Analytical Chemistry LettersRequires access

Development and Validation of RP-HPLC Method for the Estimation of Lisinopril in Tablet Dosage Form

Vikas Chander, Manindra Mohan, Richa Seth, Prashant Kumar Singh, Rakesh Singh, Sanjay Kumar Gupta

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Abstract

A new isocratic reversed-phase high performance liquid chromatographic (HPLC) method with diode-array UV detection was developed and validated for the determination of lisinopril in pharmaceutical formulation. The method validation of lisinopril was performed by using Nucleosil (125 x 4.0mm, 5mm) as stationary phase with mobile phase consisting of buffer solution, isopropyl alcohol and triethylamine (95:5:0.1) at a flow rate of 1.0 ml/min. The column temperature and spectrometric detection were monitored at 50°C and 215 nm, respectively. The 20 μl of sample was injected for the run time of 7 min. The statistical analysis of data showed that the validated method is within limits in all respective parameters and is quick and reliable for quantitative as well as quality control analysis of lisinopril for pharmaceutical formulation.

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What this paper is about

A new isocratic reversed-phase high performance liquid chromatographic (HPLC) method with diode-array UV detection was developed and validated for the determination of lisinopril in pharmaceutical formulation. The method validation of lisinopril was performed by using Nucleosil (125 x 4.0mm, 5mm) as stationary phase with mobile phase consisting of buffer solution, isopropyl alcohol and triethylamine (95:5:0.1) at a flow rate of 1.0 ml/min. The column temperature and spectrometric detection were monitored at 50°C and 215 nm, respectively. The 20 μl of sample was injected for the run time of 7 min. The statistical analysis of data showed that the validated method is within limits in all respective parameters and is quick and reliable for quantitative as well as quality control analysis of lisinopril for pharmaceutical formulation.

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Available abstract

A new isocratic reversed-phase high performance liquid chromatographic (HPLC) method with diode-array UV detection was developed and validated for the determination of lisinopril in pharmaceutical formulation. The method validation of lisinopril was performed by using Nucleosil (125 x 4.0mm, 5mm) as stationary phase with mobile phase consisting of buffer solution, isopropyl alcohol and triethylamine (95:5:0.1) at a flow rate of 1.0 ml/min. The column temperature and spectrometric detection were monitored at 50°C and 215 nm, respectively. The 20 μl of sample was injected for the run time of 7 min. The statistical analysis of data showed that the validated method is within limits in all respective parameters and is quick and reliable for quantitative as well as quality control analysis of lisinopril for pharmaceutical formulation.

Key concepts: Chemistry, Lisinopril, Chromatography, Dosage form, High-performance liquid chromatography, Triethylamine, Quantitative analysis (chemistry), Isopropyl alcohol

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Development and Validation of RP-HPLC Method for the Estimation of Lisinopril in Tablet Dosage Form — Research Paper | ScholarLens