2008•Journal of Peking University(Health Sciences)Requires access
Changes of matrix metalloproteinase 2,9 and tissue inhibitor of metalloproteinase1,2,3 expression level in aged rat lung
Jiang Li
Open publisher page 2 citations
Abstract
Objectives:To investigate the underlying mechanism of age related increase of pulmonary fibrosis incidence Methods: The levels of pulmonary collagen and TGF-β1 protein, MMP2,9 mRNA and TIMP 1,2,3 mRNA expression were measured by ELISA and realtime PCR. Results: As compared with adult rats [(756±160) pg/g vs (1 000±246) pg/g, P0.05] the aged rats had more extracellular matrix components, increased TGF-beta1 protein level, lower (3.15±1.76 vs 0.17±0.13, P0.01) MMP2 mRNA expression and TIMP 1,2,3 mRNA expressions (TIMP1 2.00±1.74 vs 0.11±0.06,TIMP2 7.60±2.51 vs 2.69±1.76, TIMP3 1.32±0.46 vs 0.29±0.16, P0.01). No difference was observed in MMP9 (1.66±0.67 vs 1.74±0.87, P0.05) mRNA expression. Conclusion: elevation in TGF-beta1 protein level, changes in the expression of MMP2/9 and decreased expression of TIMPS mRNA could be the underlying mechanism of age related increase of pulmonary incidence.