Change and significance of eNOS,MMP-1 and TIMP-1 in lung tissue of patients with CHD and pulmonary hypertension

Objective To study the change and significance of eNOS,MMP-1 and TIMP-1 expression in lung tissue of patients with congenital heart disease(CHD)and pulmonary hypertension(PH).Methods CHD patients were divided into different groups according to Heath-Edwards pulmonary vessel pathologic grading: control group(non-PH)14 cases,observation group(PHⅠ,PHⅡ,PH Ⅲ)46 cases.The eNOS mRNA,MMP-1 mRNA and TIMP-1 mRNA were detected by RT-PCR in the lung tissue of these patients.Results The levels of MMP-1 mRNA,TIMP-1 mRNA and TIMP-1 mRNA/MMP-1 mRNA were raised in observation group with control group(P0.01),eNOS mRNA was decreased in observation group with control group(P0.01).There was significant positive correlation between the MMP-1 mRNA and TIMP-1 mRNA(r=0.879,P0.01).There were significant positive correlation between MMP-1 mRNA,TIMP-1 mRNA,TIMP-1 mRNA/MMP-1 mRNA and the grade of PH(r were 0.858,0.802 and 0.315,all P0.05).There was significanthy negative correlation between the eNOS mRNA and the grade of PH(r=-0.714,P0.01).There were singificantly negative correlation between TIMP-1 mRNA,MMP-1 mRNA,TIMP-1 mRNA/MMP-1 mRNA and eNOS mRNA(r were-0.607,-0.642 and-0.777,all P0.01).Conclusion eNOS and MMP-1/TIMP-1 imbalance may contribute to pulmonary vascular remodeling in CHD with PH.