2012•Zhongguo kangfu yixue zazhiRequires access
Effects of hyperbaric oxygen on brain edema and expression of aquaporin-4 surrounding brain tissue of hemorrhagic focus following experimental intracerebral hemorrhage in two kinds of rat models
Fang Jin
Open publisher page 0 citations
Abstract
Objective:To study the effects of hyperbaric oxygen(HBO) on brain edema and expression of aquaporin-4(AQP-4) surrounding brain tissue of hemorrhagic focus following experimental intracerebral hemorrhage(ICH) in two kinds of rat models.Method:A total of 126 rats were randomly divided into five groups:sham-operated group(SHG,6 rats);control-1 group(autologous blood induced intracerebral hemorrhage model,A-ICH,30 rats),control-2 group(collagenase-induced intracerebral hemorrhage model,C-ICH,30 rats),trial-1 group(A-ICH+HBO,30 rats),trial-2 group(C-ICH+ HBO,30 rats).HBO therapy was intervened at 24h after operation,once a day.All rats were sacrificed at 24h,48h,72h,5d,7d post operation(each time point 6 rats).Brain edema and expression of AQP-4 were tested.Result:The success rates of A-ICH model and C-ICH model were 65% and 75% respectively.Brain edema content of A-ICH,C-ICH,A-ICH+HBO and C-ICH+HBO groups were significant higher than that of SHG group(P 0.05).The cerebral edema of A-ICH+HBO and C-ICH+HBO groups alleviated obviously compared with A-ICH and C-ICH groups,and the difference was significant respectively at 48h,72h,5d,7d and 48h,5d post operation(P 0.05).The brain edema of A-ICH+HBO group alleviated more early and obviously than that of C-ICH+HBO group,especially at 72h post operation(P0.05).The expression of AQP-4 appeared in all rats brain tissue,but expression of AQP-4 in SHG group was lower.That of A-ICH and C-ICH groups started rising at 24h post operation,the peak appeared at 48h,then dropped at 72h,but there were still significant different compared with SHG group at 7d post operation(P0.05).The difference between A-ICH group and C-ICH group was significant at 72h post operation(P0.05),and the difference between A-ICH+HBO group and A-ICH group was significant at all time points post operation(P0.05).but the difference between C-ICH+HBO group and C-ICH group was significant only at 48h and 72h post operation(P0.05).Compared with C-ICH +HBO group,expression of AQP-4 in A-ICH+HBO group was more obvious and early,especially at 48h and 72h post operation(P0.05).Conclusion:HBO might play neuroprotection role by relieving brain edema and down-regulating AQP-4 expression.A-ICH model was more fit for brain edema study of ICH rat.