Change of leukocyte and apoptosis at brain tissue around hematoma and their relationship with brain water content in rats with intracerebral hemorrhage

Objective To explore the change of leukocyte and apoptosis at brain tissue around hematoma and their relationship with brain water content in rats with intracerebral hemorrhage(ICH).Methods ICH models was made by nonthrombogenic autologous blood injection method in 48 rats,and then the rats were randomly divided into 8 groups: sham operation group,ICH 6 h group,ICH 12 h group,ICH 24 h group,ICH 48 h group,ICH 72 h group,ICH 1 week group and ICH 2 weeks group.The brain of rats was taken out at corresponding time point respectively,and the brain water content was measured.The HE staining and TUNEL staining was made and the leukocyte and apoptosis were counted.Results The brain water content of rats at 6 h-1 week after ICH was significantly higher than sham operation group,and which was the highest at 72 h after ICH(all P0.05).The leukocyte was not found at sham operation group,ICH 6 h group and ICH 2 weeks group.The leukocyte was interspersed and infiltrated at 12 h-1 week after ICH,and which was the most at 48 h after ICH(all P0.05).The number of apoptosis of ICH rats at each time point was significantly higher than sham operation group,and which was the highest at 72 h after ICH(all P0.05).The leukocyte and apoptosis at brain tissue around hematoma were both positively related with the brain water content(r=0.615,P0.01;r=0.578,P0.01).Conclusion The increase of leukocyte and apotosis at brain tissue around hematoma in ICH rats may be related with the formation of encephaledema.