2001•Zhonghua ganzangbing zazhiRequires access
Annexin V technique for the study of liver damage
Jiang Ji-ka
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Abstract
Objective To evaluate Annexin V technique for measuring hepatic apoptosis and to investigate the protective function of Rg1 and Rb1 on acute liver injury. Methods LPS-treated acute liver injury and the protective effect of Rg1 and Rb1 were assessed by Annexin V double staining and PI staining measurements. The activity of sPLA2 was measured by [3H]-labelled oleic acid method. Results Annexin V showed a higher sensitivity and specificity than PI staining. It was only the Annexin V assay that could discriminate normal cells, apoptosis cells, and necrotic cells. Rg1 and Rbl could reduce the percentage of hepatic apoptotic and necrotic cells (P0.01) and activity of sPLA2 (p0.01). Conclusions The Annexin V assay is an ideal method for measuring apoptosis presently. Rgl and Rbl have a definite protective effect on acute liver injury in rats.