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[Annexin V technique for the study of liver damage].

Feng Wu, Gi Chang Kang, Jinhuan Jiang

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Abstract

OBJECTIVE: To evaluate Annexin V technique for measuring hepatic apoptosis and to investigate the protective function of Rg1 and Rb1 on acute liver injury. METHODS: LPS-treated acute liver injury and the protective effect of Rg1 and Rb1 were assessed by Annexin V double staining and PI staining measurements. The activity of sPLA(2) was measured by [(3)H]-labelled oleic acid method. RESULTS: Annexin V showed a higher sensitivity and specificity than PI staining. It was only the Annexin V assay that could discriminate normal cells, apoptosis cells, and necrotic cells. Rg1 and Rb1 could reduce the percentage of hepatic apoptotic and necrotic cells (P<0.01) and activity of sPLA2 (P<0.01). CONCLUSIONS: The Annexin V assay is an ideal method for measuring apoptosis presently. Rg1 and Rb1 have a definite protective effect on acute liver injury in rats.

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OBJECTIVE: To evaluate Annexin V technique for measuring hepatic apoptosis and to investigate the protective function of Rg1 and Rb1 on acute liver injury. METHODS: LPS-treated acute liver injury and the protective effect of Rg1 and Rb1 were assessed by Annexin V double staining and PI staining measurements. The activity of sPLA(2) was measured by [(3)H]-labelled oleic acid method. RESULTS: Annexin V showed a higher sensitivity and specificity than PI staining. It was only the Annexin V assay that could discriminate normal cells, apoptosis cells, and necrotic cells. Rg1 and Rb1 could reduce the percentage of hepatic apoptotic and necrotic cells (P<0.01) and activity of sPLA2 (P<0.01). CONCLUSIONS: The Annexin V assay is an ideal method for measuring apoptosis presently. Rg1 and Rb1 have a definite protective effect on acute liver injury in rats.

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Available abstract

OBJECTIVE: To evaluate Annexin V technique for measuring hepatic apoptosis and to investigate the protective function of Rg1 and Rb1 on acute liver injury. METHODS: LPS-treated acute liver injury and the protective effect of Rg1 and Rb1 were assessed by Annexin V double staining and PI staining measurements. The activity of sPLA(2) was measured by [(3)H]-labelled oleic acid method. RESULTS: Annexin V showed a higher sensitivity and specificity than PI staining. It was only the Annexin V assay that could discriminate normal cells, apoptosis cells, and necrotic cells. Rg1 and Rb1 could reduce the percentage of hepatic apoptotic and necrotic cells (P<0.01) and activity of sPLA2 (P<0.01). CONCLUSIONS: The Annexin V assay is an ideal method for measuring apoptosis presently. Rg1 and Rb1 have a definite protective effect on acute liver injury in rats.

Key concepts: Annexin, Apoptosis, Staining, Pi, Molecular biology, Annexin A2, Medicine, Chemistry

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[Annexin V technique for the study of liver damage]. — Research Paper | ScholarLens