Antifungal activity of actinomycete strain 19G-317
Jina Li
Abstract
Jina Li
Abstract
【Objective】 The research primarily studied the antifungal activity of the fermentation product of actinomycete strain 19G-317.【Method】 The antifungal activity of the fermentation product of actinomycete strain 19G-317 was researched by mycelium growth rate,spore germination test,tissue culture method and potted plant method.【Result】 The results of mycelium growth rate test showed that the fermentation product had different inhibition effects on 22 tested plant pathogenic fungi.The inhibition rate of the fermentation broth was over 60% to 11 tested plant pathogenic fungi,and that of the extract product of mycelium was over 90% to 8 tested plant pathogenic fungi.The EC50 values of the fermentation broth and the extract product of mycelium against Sclerotinia sclerotiorum were 84.30 mg/L and 18.80 mg/L respectively.The results of spore germination test showed that the inhabition rates of the fermentation broth and extract product of mycelium were 94.26% and 96.20% to Botrytis cinerea under the concentration of 0.5 mg/mL respectively.Tissue culture method tests showed that the inhabition rates of the fermentation broth and the extract product of mycelium were 72.17% and 76.95% to Botrytis cinerea under the concentration of 10 mg/mL respectively.The results of potted test indicated that the fermentation products had both protective and therapeutic efficacy on P.capsici.【Conclusion】 These studies indicate that a potential exists for utilizing 19G-317 to control plant diseases.
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【Objective】 The research primarily studied the antifungal activity of the fermentation product of actinomycete strain 19G-317.【Method】 The antifungal activity of the fermentation product of actinomycete strain 19G-317 was researched by mycelium growth rate,spore germination test,tissue culture method and potted plant method.【Result】 The results of mycelium growth rate test showed that the fermentation product had different inhibition effects on 22 tested plant pathogenic fungi.The inhibition rate of the fermentation broth was over 60% to 11 tested plant pathogenic fungi,and that of the extract product of mycelium was over 90% to 8 tested plant pathogenic fungi.The EC50 values of the fermentation broth and the extract product of mycelium against Sclerotinia sclerotiorum were 84.30 mg/L and 18.80 mg/L respectively.The results of spore germination test showed that the inhabition rates of the fermentation broth and extract product of mycelium were 94.26% and 96.20% to Botrytis cinerea under the concentration of 0.5 mg/mL respectively.Tissue culture method tests showed that the inhabition rates of the fermentation broth and the extract product of mycelium were 72.17% and 76.95% to Botrytis cinerea under the concentration of 10 mg/mL respectively.The results of potted test indicated that the fermentation products had both protective and therapeutic efficacy on P.capsici.【Conclusion】 These studies indicate that a potential exists for utilizing 19G-317 to control plant diseases.
Key concepts: Mycelium, Botrytis cinerea, Sclerotinia sclerotiorum, Fermentation, Spore germination, Spore, Germination, Biology