2010•Acta Scientiarum Naturalium Universitatis SunyatseniRequires access

Effect of S1P5 on proliferation and migration of human esophageal cancer cells

Hu, Weimin, Yu‐Chuan Li, Ing Ing, Bao-Qian, Zhao, Yongsheng, Wang, ChaoLi, Feng, Xie, Yong-En

Abstract

AIM:To investigate the sphingosine 1phosphate (S1P) receptor expression profile in human esophageal cancer cells and the effects of S1P5 on proliferation and migration of human esophageal cancer cells. METHODS: S1P receptor expression profile in human esophageal squamous cell carcinoma cell line Eca109 was detected by semiquantitative reverse trans cription polymerase chain reaction. Eca109 cells were stably transfected with S1P5EGFP or controlEGFP constructs. The relation between the responses of cell prol...

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Key concepts: Transfection, Esophageal cancer, Cell culture, Receptor, Cancer research, Cell migration, Cancer, Cell