2000•Zhongguo mianyixue zazhiRequires access

The construction and expression of phage display scFv library from the spleen cells of mice immunized with KGla cells

Jianhua Sui, Zengxuan Song, Ming She, Liyan Zhang, Decheng Shen, Zhongchao Han

Abstract

We were able to produce a scFv library containing 3×10~(6) individual clones which showed different patterns after digested with restriction endonuclease BstN I. It indicated that the capacity and diversity of the library is sufficient for screening specific scFv. The surface display expression of the library was also verified.

About this research paper

What this paper is about

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Key concepts: Phage display, Restriction enzyme, Spleen, Molecular biology, Biology, Endonuclease, Genomic library, Virology