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Construction and preliminary selection and identification of the phage display library of the immunoglobulin repertoire of anti-human gastric cancer single chain Fv antibody

Lijuan Yang, Chengzhi Su

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Abstract

The ScFv gene library was constructed by phage display method. The affinity to antigen was detected by ELISA after panning. Eighteen antigen-binding clones were identified from 94 individual phagemid clones.

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The ScFv gene library was constructed by phage display method. The affinity to antigen was detected by ELISA after panning. Eighteen antigen-binding clones were identified from 94 individual phagemid clones.

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Available abstract

The ScFv gene library was constructed by phage display method. The affinity to antigen was detected by ELISA after panning. Eighteen antigen-binding clones were identified from 94 individual phagemid clones.

Key concepts: Phage display, Antibody, Cancer, Antibody Repertoire, Selection (genetic algorithm), Repertoire, Identification (biology), Immunoglobulin light chain

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Construction and preliminary selection and identification of the phage display library of the immunoglobulin repertoire of anti-human gastric cancer single chain Fv antibody — Research Paper | ScholarLens