Studies of Yellow-seeded Gene in Brassica napus with mRNA Differential Display

A repetitive and steady differential cDNA fragment was obtained by screening 90 pairs of random primers and anchor primer combination by using mRNA differential display technique in two groups of different origin NIL of yellow-seeded Brassica napus. Northern bloting confirmed the result by using the differential cDNA fragment as probe, and sepeculated that the differential cDNA fragment might be related to the color gene of Brassica napus. The differential cDNA fragment was cloned and sequenced and two nesting primers was designed to extend the fragment by PCR-Walking technique. The sequence analysis revealed that the cloned cDNA fragment might be related to the development of Brassica napus seed color. Some problems in mRNA differential display were discussed.