Cloning and Sequencing of a cDNA Sequence Encoding FAD2 from Brassica napus

To increase the oleate contents in rape and breed a rape variety of high oleate contents,the first strand of cDNA from the total RNA extracted from leaf of rapeseed( Brassica napus cv XY15) was synthsized and was used as a template for PCR reaction with oligonucleotide as a primer synthesized according to the FAD2 gene from oilseed rape. The PCR products were cloned into pGEM T Easy and sequenced. The results show that the cloned fragment contains 654 nucleotides,and shares a homology of 100% in comparison part with the reported data.