Molecular cloning of human IL-7cDNA and construction of eukaryotic vector expressing hIL-7

Objective: To construct a vector expressing eukaryotic human interluken 7 ( hIL 7 ). Methods: hIL 7 DNA was identified and cloned (cDNA) from human spleen tissue using reverse transcription polymerase chain reaction (RT PCR). We incorporated the cDNA into the pMD18 T plasmid. The pMD18 T plasmid was then inserted into a dual expression vector (prokaryotic and eukaryotic) pBK CMV and called pBK CMV hIL 7 .We used pBK CMV hIL 7 vector to infect E.coli DH5α. The expression of the recombinant hIL 7 protein (rhIL 7) by E.coli DH5α was analyzed using SDS PAGE and western blot testing. Results: The genetically engineered E.coli DH5α did express rhIL 7 confirmed by western blot. Conclusion: The successful construction of a genetically engineered eukaryotic gene for hIL 7 was done. This will enable further research into therapeutic uses for hIL 7.