The Cloning and Expression of cDNA Encoding Human IL-17

Objective To obtain recombinant human interleukin 17(hIL?17) for science research and application.Methods The complete encoding region of hIL?17 gene was cloned from activated human peripheral blood mononuclear cells by RT?PCR and a recombinant plasmid pBS?hIL?17 was constructed for sequencing.Then hIL?17 cDNA was subcloned into vector pBV220 to express hIL?17 protein in E.Coli XL1?Blue.Results The hIL?17cDNA was cloned and the hIL?17 protein was highly expressed in E.Coli up to 39.7% of total bacterial protein by temperature inducing.Conclusion The hIL?17cDNA was cloned successfully and hIL?17 protein can be highly expressed in E.Coli by temperature inducing.