Distribution of Genotypes of Alcohol Dehydrogenase 2 and Aldehyde Dehydrogenase 2 in Japanese Twin Children

Objective In order to prevent alcohol related deseases, this study investigated the distribution of the genes controlling alcohol metabolism in Japan′s twin. Methods Restriction fragment length polymorphism polymerase chain reaction (RFLP PCR) technique was used to measure the control gene of alcohol metabolized enzymes and the genotypes of alcohol dehydrogenase 2 (ADH2) and aldehyde dehydrogenase 2 (ALDH2), which were distributed in Japan′s twins. At the same time, according to the difference in genotypes, the sensitive individuals were screened from the study subjects. Results The distribution of ADH2 and ALDH2 genes were conistent with the Hardy weinberg equation. The three genotypes of ADH2 gene were ADH2 1/ADH2 1(1.1%), ADH2 1/ADH2 2(44.6%) and ADH2 2/ADH2 2(54.3%). And those of ALDH2 gene were ALDH2 1/ALDH2 1(41 3%), ALDH2 1/ALDH2 2(39.1%) and ALDH2 2/ALDH2 2 (19.6%). The frequency of ADH2 and ALDH2 genes was 0.255, 0.745 and 0.609, 0.391 respectively. Conclusion Not only the distribution of genotypes of ADH2 and ALDH2 is known, but also the sensitive individuals are found, which can help prevent alcohol related disease.