The enhancement effect of CD59 on CD55-mediated T cell signal transduction

AIM:To study the enhancement effect of glycosylphosphatidyl inositol(GPI)-anchored protein CD59 on CD55-mediated T cell signal transduction.METHODS:Human Jurkat cells were divided into 3 groups:Jurkat cells(Ⅰgroup),Jurkat cells transfected with blank plasmid(Ⅱgroup)and Jurkat cells transfected with siRNA plasmid(Ⅲ group).CD59 mRNA level was detected by RT-PCR.The cell proliferation activity of the three groups was measured by MTTcolorimetry after crosslinking anti-CD55 mAb and anti-CD59 mAb.The phosphorylation levels of Src family of protein tyrosine kinase(PTK) were investigated by immunoblot analysis,and the kinetic changes of [Ca2+] in T cell endochylema were determined by Laser scanning confocal microscope imaging.RESULTS:CD59 expression was successfullyinhibited in Ⅲgroup cells after transfecting.The cell proliferation,phosphorylation levels of Src family of PTK and degree of [Ca2+] inⅠandⅡgroups were increased compared with Ⅲ group(P0.05),after crosslinking of CD55 and CD59 antibodies,but there was no difference between Ⅰ group and Ⅱ group.CONCLUSION:CD59 can enhance the effect of CD55-mediated T cell signal transduction.