Specific Inhibition of CD147 Gene Expression in Jurkat T Lymphocytes Decreased Colony Forming Capability

Objective To investigate the effect of inhibiting CD147 gene expression by small interfering RNA(siRNA) in Jurkat T lymphocytes on the colony forming capability.Methods Specific siRNA targeting CD147 gene was transfected into Jurkat cells by LipofectimineTM 2000.The changes of CD147 mRNA level were determined by semi-quantitative RT-PCR,and total proteins level and cell surface protein were respectively detected by Western blot and flow cytometry at 48 h after transfection.By fluorescence inverted microscope,the changes of colony forming in Jurkat cells between the specificly inhibit ed CD147 group and the normal group were compared.Results Compared with control group,the CD147 mRNA and protein level in siRNA interference group were reduced by(38.57±1.55)% and(47.4±1.47)% respectively,and the mean fluorescence intensity of CD147 in Jurkat cells was descended by(50.5±4.7)%.Along with the inhibition of CD147,the colony forming of siRNA interference group obviously reduced(P0.05).Conclusion siRNA can specifically inhibit the expression of CD147 in Jurkat cells,which can decrease the colony forming of the Jurkat cells,indicating that CD147 may potentially facilitate inflammatory cell adhesion and aggregation.