Effects of XUEBIJING injection on hepatic gelsolin and inflammatory cytokine in septic rats

Objective To observe the effects of XUEBIJING injection on the expression of hepatic gelsolin(GSN) and inflammatory cytokine in septic rats.Methods Seventy-eight male Wistar rats were involved in present study and randomly divided into normal control group(n=6),sham operation group(n=24),cecal ligation puncture(CLP) group(n=24) and XUEBIJING group(n=24).The CLP sepsis models of rats were established with the animals in the latter two groups.XUEBIJING injection was injected intravenously at 2,12,24,36,48 and 60 hours after operation(4ml/kg each) in XUEBIJING group,while rats in sham operation group and CLP groups received same amount of normal saline by intravenous injection.The levels of hepatic GSN and high mobility group box-1(HMGB1) protein at 6,12,24 and 72 hours after operation were determined by ELISA,and limulus test was performed to measure the hepatic LPS levels.Results Compared with normal control and sham operation group,The hepatic GSN levels decreased and LPS and HMGB1 levels increased markedly in CLP groups(P0.05).Compared with CLP group,the hepatic GSN levels increased and LPS and HMGB1 levels decreased markedly in XUEBIJING group(P0.05).Correlation analysis revealed that a significant negative correlation existed between the hepatic GSN contents and LPS levels at each of time points in CLP group(P0.05),while no obvious correlation was found between GSN and HMGB1 levels(P0.05).Conclusion XUEBIJING injection may enhance the production of hepatic GSN via inhibition of LPS in septic rats,thereby having beneficial effect on septic response.