Rapid determination of fruto-oligosaccharides content in probiotic powder by high performance liquid chromatography-evaporative light-scattering detector

Objective To establish a rapid determination for the rapid separate kestose, nystose and 1F-fructofuranosyl nystose by high performance liquid chromatography-evaporative light-scattering detector(HPLC-ELSD) method, and the concentration of fruto-oligosaccharides can be determined in probiotics powder. Methods After pretreatment of samples, acetonitrile and water as the mobile phase, while the elution method was gradient elution, the samples were separated by XAmide(4.6 mm×250 mm,5 μm) neutral amide chromatographic column, and detected by evaporative laser detector. Results The linear ranges of kestose, nystose and 1F-fructofuranosyl nystose were from 0.2671 to 3.168 mg/m L, 0.2429 to 2.8215 mg/m L and 0.1617 to 3.544 mg/m L, respectively, and the linear correlation coefficient r0.99. The recovery rate of the 3 compositions was between 97.0%~105.0%. Conclusion This method can effectively separate the kestose, nystose and 1F-fructofuranosyl nystose. It is a high generalization, rapid, simple and accurate method, and can be applied for the determination of fruto-oligosaccharides in probiotics powder.