2006•Korean Journal of Food Science and TechnologyRequires access

Determination of Fructooligosaccharides and Raffinose in Infant Formula by High Performance Liquid Chromatography with Evaporative Light Scattering Detector

Man-Sub Shin, Jaewoo Park, Mi-Ran Cho, Sung-Ok Song, Chun-Sun Kim, Chun-Bae Choi, Seoung-Won Lee, Ki-Woong Lee, Chi-Hoon Chang, Byung‐Man Kwak

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Abstract

A method was developed for the determination of fructooligosaccharides and raffinose contents in infant formula. The samples were extracted and analyzed by liquid chromatography equipped with carbohydrate column and evaporative light scattering detector. The mobile phase used for the gradient mode was water-acetonitrile, at a flow rate of 1.0mL/min. The method showed a mean recovery of 95-99%, the relative standard deviation obtained in the precision study was 0.774-8.982%, the quantification and detection limits were 25-50mg/L.

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Key concepts: Raffinose, Chromatography, Chromatography detector, Chemistry, Detector, Relative standard deviation, Infant formula, Analytical Chemistry (journal)