2012•Journal of Emergency in Traditional Chinese MedicineRequires access
Experimental study of Huangqi Shuizhi Preparation on Expression of TGF-β1 of Renal Tissue in STZ-induced Diabetic Nephropathy Rats
Xiong Rong-bin
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Abstract
Objective: To investigate the effect of Huangqi shuizhi Preparation on expression of TGF-β1 of renal tissue in STZ-induced diabetic nephropathy rats,and further explore the mechanism of action of Huangqi shuizhi Preparation on the renal protection in STZ-induced Diabetic Nephropathy Rats.Methods: Fed with high fat diet and STZ produced by intraperitoneal injection in rats with diabetic nephropathy.Rats were randomly divided into six groups: normal control group,model control group,Chinese medicine group(Huangqi shuizhi Preparation group),Leigongtengpian Duo DAI pian group,western medicine group(Lotensin group)and Chinese and Western medicine group(Huangqi shuizhi Preparation and Lotensin group).Rats were determined after treatment fasting blood glucose.Leave all rats's urine before the end of the experiment.And the 24 h urinary protein was determined.The expression of TGF-β1 of renal tissue in STZ-induced diabetic nephropathy rats was measured with immunohistochemical method.Results: After treatment for two months with renal sugar granule,the general situation in rats compared with the model rats were improved better;and the weight also gain significantly.Fasting blood glucose,24h urinary protein in the treatment groups were significantly lower than the model group;and TGF-β1 of Renal Tissue in the model rats showed high expression,respectively significantly higher than every treatment groups.Conclusion: The possible mechanism of the renal protective effect of Leigongtengpian Duo DAI pian on STZ-induced Diabetic Nephropathy Rats is the inhibited expression of TGF-β1 in renal tissue in rats.