EXPRESSION OF P16 AND CYCLIN D1 IN THE COURSE OFCARCINOGENESIS OF THE STOMACH

Objective: To determine p16 and cyclin D2 expression in the specimen of gastric carcinoma, atypichyperplasia, atrophic gastritis, superficial gastritis andnormal gastric mucosa. Methods: Using immunohistochemical method (ABC), the samples of 58 adenocarcinomas, 22 atypic hyperplasias, 28 atrophic gastritis,27 superficial gastritis and 15 gastric epitheliums wereanalyzed. Results: Positive immunostaining rate forp16 protein was the highest in normal gastric mucosaand decreased with the lesions progressing fromsuperficial gastritis to atrophic gastritis to atypitalhyperplasia and to adenocarcinoma (85 %, 78.6 %, 31.8 %,48.3% respectively); Positive immunostaining of cyclinD1 can observed in atrophic gastritis. With the lesionsprogressing from atrophic gastritis to atypicalhyperplasia to adenocarcinoma, its expression rateincreased (17.9%, 36.4%, 53.4 % respectively), and therewas a significant difference between adenocarcinomaand atrophic gastritis group (P0.05). An interestingobservation was that inverse expression between p16and cyclin D1, was shown in most of gastric cancerdetected. Conclusion: It is indicated that p16 andcyclin DI play an important role in the gastriccarcinogenesis, the inverse expression between p16 andcyclin DI suggested that there is a suppression trend inthem.