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2019•Unpublished venueOpen access

ABTS decolorization assay – in vitro antioxidant capacity v1

Daniel C. Moreira

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Abstract

This protocol describes how to perform the ABTS decolorization assay to assess potential in vitro antioxidant capacity of molecules and extracts using microtiter plates. Procedures are based on the method described in R. Re, N. Pellegrini, A. Proteggente, A. Pannala, M. Yang, C. Rice-Evans, Antioxidant activity applying an improved ABTS radical cation decolorization assay, Free Radic. Biol. Med. 26 (1999) 1231–1237.

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This protocol describes how to perform the ABTS decolorization assay to assess potential in vitro antioxidant capacity of molecules and extracts using microtiter plates. Procedures are based on the method described in R. Re, N. Pellegrini, A. Proteggente, A. Pannala, M. Yang, C. Rice-Evans, Antioxidant activity applying an improved ABTS radical cation decolorization assay, Free Radic. Biol. Med. 26 (1999) 1231–1237.

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Available abstract

This protocol describes how to perform the ABTS decolorization assay to assess potential in vitro antioxidant capacity of molecules and extracts using microtiter plates. Procedures are based on the method described in R. Re, N. Pellegrini, A. Proteggente, A. Pannala, M. Yang, C. Rice-Evans, Antioxidant activity applying an improved ABTS radical cation decolorization assay, Free Radic. Biol. Med. 26 (1999) 1231–1237.

Key concepts: ABTS, Antioxidant, Antioxidant capacity, In vitro, Chemistry, In vitro toxicology, Biochemistry, Chromatography

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