Lipase producing thermophilic bacteria isolation and characterization from hot springs of Central India.

Hot springs considered being a unique domain of novel extremophiles which are useful for production of industrially important enzymes, to understand the metabolic reactions and for producing other biotechnological products.The aim of the present research was isolation and characterization of thermophilic bacteria containing potential to produce extracellular lipase enzyme from the geothermal springs located in central India.Water is used as test sample in order to obtain thermophilic bacteria.10 thermophilic bacteria were isolated from the water samples and subjected to screening for the potential of producing lipase enzyme of which 8 isolates are potential producer of extracellular lipase.Two isolates with higher zone of clearance around them were selected for the further investigation.Morphological, biochemical and molecular identification by 16S rRNA sequencing of these isolates (BAC23 and BAC26) could be identified as Bacillus haynesii and Bacillus clausii.The lipolytic activity of these isolates was recorded stable at optimum temperature range of 45°C-60°C with alkaline pH at 9.0-9.4 and at 0.5-1.0M salinity.Capability to produce extracellular thermostable lipase at high temperature and pH, these strains of Bacillus spp.can be presented as promising contender for modern industrial applications.