Anti-angiogenic Effect of Black Rice Bran (Oryza Sativa L. Sembada Hitam) on Soluble Fms-Like Tyrosine Kinase and Placental Growth Factor in Preeclampsia

Preeclampsia is a multisystem disorder of pregnancy that contributes to morbidity and mortality worldwide. Soluble FMS-like tyrosine kinase-1 (sFlt-1) is considered as an etiologic factor of endothelial damage in preeclampsia. Imbalance of sFlt-1 and PlGF could cause failure in trophoblastic invasion and physiological remodeling of spiral artery, and ultimately placental hypoxia. This study aimed to observe anti-angiogenic properties of black rice bran on preeclampsia through measurement of sFlt-1 and PIGF. Serum was collected from pregnant women at 28-34 weeks of gestational age with preeclampsia, and normal preeclampsia as control. Level sflt-1 and PGIF was quantified with enzyme linked immunosorbent assay (ELISA). Absorbance was read at 450 nm wavelength. Extract of black rice bran has anti-angiogenic properties by significantly decreasing sFlt-1 as well as increasing PIGF in preeclampsia-induced HUVEC. This makes black rice bran a promising agent which can be further used in preeclampsia treatment.