Determination of 11 kinds of ginsenosides from Ginseng Bud by RP-HPLC

Objective: To establish a method for the determination of 11 kinds of ginsenosides from both fresh and dried Ginseng Bud. Methods: RP- HPLC was conducted on a Cosmosil 5 C18- MS column( 250 mm × 4. 6 mm,5μm) with the mobile phase of acetonitrile- 0. 05 mol·L- 1potassium dihydrogen phosphate solution by gradient elution at a flow rate of 1 m L·min- 1. The column temperature was maintained at 25 ℃,and the detection wave length was 203 nm. Results: The linear response ranged from 18. 83-301. 20 μg·m L- 1for malonyl ginsenoside Rb1( r =0. 999 1),19. 01-304. 20 μg·m L- 1for malonyl ginsenoside Rb2( r = 0. 999 8),18. 94-303. 00 μg·m L- 1for malonyl ginsenoside Rc( r = 0. 999 3),18. 79-300. 60 μg·m L- 1for malonyl ginsenoside Rd( r = 0. 999 4),18. 56-297. 00 μg·m L- 1for ginsenoside Rb1( r = 0. 999 0),18. 86-301. 80 μg·m L- 1for ginsenoside Rc( r = 0. 999 1),18. 94-303. 00 μg·m L- 1for ginsenoside Rb2( r = 0. 999 5),37. 58-601. 20 μg·m L- 1for ginsenoside Rd( r =0. 999 6),37. 58-601. 20 μg·m L- 1for ginsenoside Re( r = 0. 999 1),19. 05-304. 80 μg·m L- 1for ginsenoside Rg1( r = 0. 999 3) and 18. 68-298. 80 μg·m L- 1for ginsenoside Rg2( r = 0. 999 6). The average recoveries( n = 6)were between 98. 3%-102. 9%,and the RSD ≤3. 0%. In fresh Ginseng Bud,the contents of 11 kinds of ginsenosides was 10. 80%,and the content of malonyl ginsenosides was 3. 46%; in dried Ginseng Bud,the contents of 11 kinds of ginsenosides was 10. 68%,and the content of the malonyl ginsenosides was 1. 21%. Conclusion: The established method is simple,fast and accurate,and can be applied for the determination of the content of 11 kinds of ginsenosides from both fresh and dried Ginseng Bud.