EFFECT OF GRAPE SEED EXTRACT AND POWDER ON OXIDANT STRESS IN KIDNEY AND LIVER INJURY BY ALCL3

The work aimed antioxidant properties of grape seed extract (GSE) against liver and kidney injury caused by ALCL3 in experimental rats. Twenty-five male Sprague-Dawley rats weighing (155 ± 5g) were used for 4 weeks. Rats classified into five groups (5 rats). The first group was used as a negative control and fed on basal diet only. The other four groups were administered ALCL3 at a single dose of 34 mg/kg / bwt through the drink water to induce liver and kidney injury. One of these groups left as positive control (group 2). The third group was treated with 50 mg grape seed proanthocyanidin extract /kg /bwt day. The fourth group was treated with 10 % grape seed proanthocyanidin powder /kg / diet day. The fifth group was treated with 50 mg grape seed proanthocyanidin extract /kg / bwt day and 10% grape seed proanthocyanidin powder /kg / diet day. The results showed that significant decrease in plasma and hepatic content analysis of grape seed extract and inhibited the levels of liver injury biomarkers (AST and ALT), also improved the kidney function enzymes. These results suggested that grape seed extract reduce organ injury through its ability to balance the oxidant–antioxidant status. This may be mediated by multiple pathways, including decreasing the oxidative stress and the great role of proanthocyanidin as a powerful antioxidant. Key words: Grape Seed - Oxidant–Antioxidant Status - ALCL3 - Ischemia