RP-HPLC determination for the content and related substances of triamcinolone acetonide acetate and chloramphenicol solution

Objective:To establish an RP-HPLC method for determination of the content and related substances of triamcinolone acetonide and chloramphenicol solution.Methods:Dionex C18(250 mm×4.6 mm,5 μm) column was adopted,the mobile phase consisted of 0.1 mol· L-1-heptanesulfonic acid sodium salt(including 0.1 mol·L-11-heptanesulfonic acid sodium salt,0.5 mol·L-1patassiumdihydrogen and 0.5% trithylamine,adjust pH=2.5 with phosphoric acid)(A)-methanol(B)with gradient elution [0 min(66%A)→20 min(66%A) →30 min(35%A)→49 min(35%A) →50 min(66%A) →60 min(66%A)] at the flow rate of 1.0 mL·min-1;the detection wavelength was 277 nm during 0-20 min and 230 nm during 20-60 min.Results:The linear response ranges of chloramphenicol,triamcinolone acetonide acetate and naphazoline hydrochloride were 30.375-3.000 mg·mL-1(r=0.9990),0.0127-0.1016 mg·mL-1(r=0.9989)and 0.0262-0.2096 mg·mL-1(r=0.9992).Conclusion:The result of determination is accurate and specific.This method is suitable for determination of the content and related substances of Triamcinolone Acetonide Acetate and Chloramphenicol solution.