Enhanced production of tannase through response surface methodology and its partial purification from fungal isolate (TPF-13)

For various industrial purposes, an important enzyme Tannin acyl hydrolase (tannase) is used to catalyse the reaction by breaking up of the ester and depside bonds in tannin rich products. In present studies, screening of tannase yielding fungal strains was done and isolated strain was marked as tannase producing fungus (TPF-13). It was a hyper-producer strain that produced extracellular tannase under submerged fermentation using tannic acid powder. By using Response surface methodology, the culture conditions for best tannase production were optimized which included the effects of different parameters on TPF-13. Maximum tannase activity (346 U/ml) was obtained in medium supplemented with 0.05% each of K2HPO4, KH2PO4, MgSO4.7H2O, 0.3% NH4NO3, 0.03% CaCl2 and 7% tannic acid at 30 oC and pH 7 after 72 hours. The specific activity of 1.25 U/mg with a yield of 0.007 per cent was recorded from partially purified tannase with 0.25 fold.