2020•Stem Cell ResearchOpen access

Generation and characterization of two induced pluripotent stem cell lines (NUIGi038-A, NUIGi038-B) from dermal fibroblasts of a healthy individual

Ning Ge, Min Liu, Xiaoran Zhu, Janusz Krawczyk, Veronica McInerney, Sanbing Shen, Timothy O’Brien, Terence Prendiville

Abstract

Two human induced pluripotent stem cell (hiPSC) lines (NUIGi038-A, NUIGi038-B) were generated from dermal fibroblasts of a healthy 47 year old female using non-integrational Sendai reprogramming method expressing OCT4, SOX2, KLF4 and C-MYC. Characterization of both hiPSC lines was confirmed by the expression of typical pluripotency markers and differentiation potential in vitro.

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Key concepts: Induced pluripotent stem cell, KLF4, SOX2, Biology, Reprogramming, Cell biology, Cell culture, In vitro