2020IntechOpen eBooksOpen access

Cost-Effective Technical Tips for Agarose Gel Electrophoresis of Deoxyribonucleic Acid

Noboru Sasagawa

Open full text 1 citations

Abstract

Agarose gel electrophoresis is one of the most fundamental experiment in biochemistry and/or molecular biology, especially in analyzing deoxyribonucleic acid (DNA) or ribonucleic acid (RNA). Many laboratories do agarose gel electrophoresis almost every day. Besides, sometimes we need to prepare tens of agarose gels at a time for training and/or practices of students. In such situations, the more cost-effective way we have, the much more experiments in laboratories/trainings of students we can achieve. Actually, experiments of using agarose can be achieved in a more inexpensive way. In this manuscript, conditions of agarose gel electrophoresis experiment (agarose, buffer, and equipment) are considered, and achievements of such efforts are described.

Open-access reader

About this research paper

What this paper is about

Agarose gel electrophoresis is one of the most fundamental experiment in biochemistry and/or molecular biology, especially in analyzing deoxyribonucleic acid (DNA) or ribonucleic acid (RNA). Many laboratories do agarose gel electrophoresis almost every day. Besides, sometimes we need to prepare tens of agarose gels at a time for training and/or practices of students. In such situations, the more cost-effective way we have, the much more experiments in laboratories/trainings of students we can achieve. Actually, experiments of using agarose can be achieved in a more inexpensive way. In this manuscript, conditions of agarose gel electrophoresis experiment (agarose, buffer, and equipment) are considered, and achievements of such efforts are described.

Why it matters

OpenAlex reports 1 citations for this work. Citation counts describe recorded attention and do not establish research quality.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Agarose gel electrophoresis is one of the most fundamental experiment in biochemistry and/or molecular biology, especially in analyzing deoxyribonucleic acid (DNA) or ribonucleic acid (RNA). Many laboratories do agarose gel electrophoresis almost every day. Besides, sometimes we need to prepare tens of agarose gels at a time for training and/or practices of students. In such situations, the more cost-effective way we have, the much more experiments in laboratories/trainings of students we can achieve. Actually, experiments of using agarose can be achieved in a more inexpensive way. In this manuscript, conditions of agarose gel electrophoresis experiment (agarose, buffer, and equipment) are considered, and achievements of such efforts are described.

Key concepts: Agarose, Agarose gel electrophoresis, Gel electrophoresis of nucleic acids, Electrophoresis, DNA, Gel electrophoresis, Chromatography, Color marker

Related papers

Back to paper searchBrowse research topicsOriginal source
Cost-Effective Technical Tips for Agarose Gel Electrophoresis of Deoxyribonucleic Acid — Research Paper | ScholarLens