Cost-Effective Technical Tips for Agarose Gel Electrophoresis of Deoxyribonucleic Acid
Noboru Sasagawa
Abstract
Open-access reader
Noboru Sasagawa
Abstract
Open-access reader
Agarose gel electrophoresis is one of the most fundamental experiment in biochemistry and/or molecular biology, especially in analyzing deoxyribonucleic acid (DNA) or ribonucleic acid (RNA). Many laboratories do agarose gel electrophoresis almost every day. Besides, sometimes we need to prepare tens of agarose gels at a time for training and/or practices of students. In such situations, the more cost-effective way we have, the much more experiments in laboratories/trainings of students we can achieve. Actually, experiments of using agarose can be achieved in a more inexpensive way. In this manuscript, conditions of agarose gel electrophoresis experiment (agarose, buffer, and equipment) are considered, and achievements of such efforts are described.
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Agarose gel electrophoresis is one of the most fundamental experiment in biochemistry and/or molecular biology, especially in analyzing deoxyribonucleic acid (DNA) or ribonucleic acid (RNA). Many laboratories do agarose gel electrophoresis almost every day. Besides, sometimes we need to prepare tens of agarose gels at a time for training and/or practices of students. In such situations, the more cost-effective way we have, the much more experiments in laboratories/trainings of students we can achieve. Actually, experiments of using agarose can be achieved in a more inexpensive way. In this manuscript, conditions of agarose gel electrophoresis experiment (agarose, buffer, and equipment) are considered, and achievements of such efforts are described.
Key concepts: Agarose, Agarose gel electrophoresis, Gel electrophoresis of nucleic acids, Electrophoresis, DNA, Gel electrophoresis, Chromatography, Color marker