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Agarose Gel Electrophoresis of Nucleic Acids

Denise Williams, Ralph Rapley

Open publisher page 6 citations

Abstract

DNA fragments may be separated by gel electrophoresis in a gel composed of agarose. This allows DNA fragments to be resolved on the basis of their molecular weight. The percentage of agarose used depends on the size of fragments to be resolved. In general a 0.8–1% gel may be used for effective separation of DNA fragments of 100–1500 base pairs ( 1 ). These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

About this research paper

What this paper is about

DNA fragments may be separated by gel electrophoresis in a gel composed of agarose. This allows DNA fragments to be resolved on the basis of their molecular weight. The percentage of agarose used depends on the size of fragments to be resolved. In general a 0.8–1% gel may be used for effective separation of DNA fragments of 100–1500 base pairs ( 1 ). These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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OpenAlex reports 6 citations for this work. Citation counts describe recorded attention and do not establish research quality.

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Available abstract

DNA fragments may be separated by gel electrophoresis in a gel composed of agarose. This allows DNA fragments to be resolved on the basis of their molecular weight. The percentage of agarose used depends on the size of fragments to be resolved. In general a 0.8–1% gel may be used for effective separation of DNA fragments of 100–1500 base pairs ( 1 ). These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Key concepts: Agarose, Gel electrophoresis of nucleic acids, Nucleic acid, Agarose gel electrophoresis, DNA, Gel electrophoresis, Chemistry, Electrophoresis

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