2009•World Applied Sciences JournalRequires access

Determination of Genetic Relations among Four Salvia L. Species Using RAPD Analysis

Soodabeh Saeidnia, Zargham Sepehrizadeh, Ahmad Reza Gohari, Elham Jaberi, Golam Reza Amin, A Hadjiakhoondi

Abstract

The phylogenic relationship among four species of Salvia (S. macrosiphon Boiss., S. Aethiopis L., S. brachyantha (Bordz.) Pobed. and S. sclarea L.) and one species of Nepeta (N. saccharata Bunge) was investigated by Randomly Amplyfied Polymorphic DNA analysis (RAPD). All the species were clearly identified using 20 primers tested (Z1- Z20). The total number of amplification products produced with these primers was (548). Genetic distance was calculated in order to construct phylogenic dendrogram of closely related samples. Results indicated that N. saccharata Bunge had the closest relationship with two samples of S. macrosiphon and S. Aethiopis. The species S. brachyantha have not only showed farther genetic distance from S. macrosiphon and S. Aethiopis but also different patterns of RAPD-PCR compare to others. Dendrogram showed one main cluster included five tested species of Salvia and Nepeta .

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Key concepts: Dendrogram, RAPD, Genetic distance, Biology, Nepeta, Salvia, Genetic relationship, Botany