RAPD study on inter-species relationships in Calligonum
Jun Ren
Abstract
Jun Ren
Abstract
species of genus Calligonum were analyzed using random amplified polymorphic DNA (RAPD) markers.To assess the relationships among species,16 random decamer oligonucleotide primers from Sangon were used for polymorphic selection.A total of 45 bands amplified from 3 primers were selected for RAPD analyses.The data were used to generate simple matching coefficients and to construct a dendrogram using UPGMA in STATISTICA programs.The results showed that:(1) Distinct genetic differences and extensive genetic diversity were present among the species.(2) 14 species were clustered into 4 groups,this is basically consistent with the results of traditional morphological taxonomy.
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species of genus Calligonum were analyzed using random amplified polymorphic DNA (RAPD) markers.To assess the relationships among species,16 random decamer oligonucleotide primers from Sangon were used for polymorphic selection.A total of 45 bands amplified from 3 primers were selected for RAPD analyses.The data were used to generate simple matching coefficients and to construct a dendrogram using UPGMA in STATISTICA programs.The results showed that:(1) Distinct genetic differences and extensive genetic diversity were present among the species.(2) 14 species were clustered into 4 groups,this is basically consistent with the results of traditional morphological taxonomy.
Key concepts: RAPD, UPGMA, Dendrogram, Biology, Genetic diversity, Taxonomy (biology), Genetics, Genetic variation