Comparison of single-layer anastomosis and double-layer anastomosis of small intestine in dogs

Objective To evaluate the effectiveness and safety of single-layer anastomosis and double-layer anastomosis of small intestine.Methods Twelve dogs were divided into two groups:group A ( single-layer anastomosis at small intestine 100 cm after Treitz ligament and double-layer anastomosis at small intestine 200 cm after Treitz ligament,n =6) ; group B (double-layer anastomosis at small intestine 100 cm after Treitz ligament and single-layer anastomosis at small intestine 200 cm after Treitz ligament,n =6).The time used for each anastomosis were recorded.The second operations were performed on postoperative day 7 on all the dogs to determine in situ anastomostic bursting pressures (ABP) and the pressures while the serosa layer was tom.Results No anastomosis leak was found in twenty-four stoma of the twelve dogs.There was no significant difference between the stoma created by single-layer anastomosis and double-layer anastomosis on intraperitoneal adhesions.The averaged ABP in the single-layer anastomosis group and the double-layer anastomosis group was (325.83 ± 88.03) and (331.25 ± 70.33) cm H2O ( 1 cm H2O =0.098 kPa,P ＞ 0.05 ).The pressures while the serosa layer was torn in the two group was ( 185.42 ± 40.87 ) and ( 182.08 ± 20.72) cm H2O respectively ( P ＞ 0.05 ).The time used for single-layer anastomosis and double-layer anastomosis was ( 17.08 ± 3.20 ) and (23.50 ± 2.50 ) minutes respectively (P＜0.01 ).Conclusion The single-layer anastomosis is an effective and safe method for the anastomosis of small intestine. Key words: Small intestine ;  Anastomosis;  Anastomosis leak;  Dogs