Experiment study of single-layer anastomosis in clinical

[Objectives] To study single-layer anastomosis is feasible and safe in the gastrointestinal tract reconstructive. [Methods] 24 New Zealand rabbits were randomly divided into A group (single-layer anastomosis group, 12 rabbits) and B group (two-layer anastomosis, 12 rabbits), which were evenly matched according to sex, weight. A group performed single-layer anastomosis end to end gastrointestinal tract reconstructive, while B performed end to end gastrointestinal tract reconstructive. And the 2nd, 4 th, 7 th day after the surgery, we measured the stomas inner diameter, carried out the pressure experiment, and observed the new formed granulation tissue in light microscope, Election microscope and immunohistochemistry (IH) section. [Result] The results showed that there was no significant statistical differences in pressure-proof, inner diameter and pachy-wall between single-layer anastomosis and two-layer anastomosis (P 0.05). No anastigmatic bleeding developed in all rabbits. [Conclusion] There is no significant statistical difference between safety between A group and B group, but after single-layer anastomosis end to end gastrointestinal tract reconstructive performed, the granulation tissue developed quickly, and cost less operation time and expenditure,we concluded that the single-layer anastomosis is a safe, reliable suture.