2015•Zhonghua shiyan waike zazhiRequires access
Protective effects of limbs ischemic preconditioning on lung ischemia-reperfusion injury in rats
Leit-ing Liu, Yan Tan, Yin Gui-lin, Shui-Bo Zhu, Zange Hu, Xuhui Gao
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Abstract
Objective
To evaluate the effects of limbs ischemic preconditioning on lung ischemiareperfusion injury in rats.
Methods
Twenty-four male healthy SD rats were randomly divided into 3 groups(n= 8 each):sham group(S group),ischemia-reperfusion group(I/R group)and limbs ischemic preconditioning group(LIPER group). In S group, animals underwent a sham thoracotomy and then the rats were observed for 165 min. In I/R group, the left hilum of lung was occluded with a noncrushing microvascular clamp for 45 min, followed by 120 min reperfusion. In LIPER group, the period of lung ischemia was held constant at 45 min, followed by 120 min reperfusion,and the rats underwent 4 episodes of 5 min legs ischemia at 5 min intervals after lung occlusion for 5 min.Arterial blood samples were taken for blood gas analysis at 120 min of reperfusion. Rats were killed at 120 min reperfusion and lungs were removed for determination of malondialdehyde(MDA)content, lung wet/dry weight ratio(W/D),superoxide dismutase(SOD)and myeloperoxidase(MPO)activities.The lung tissues were subjected to histological examination under a light microscrope,and lung injury was sored.
Results
At the end of reperfusion, as compared with I/R group, acute lung injury scores in LIPER group were significantly reduced[(6.75± 0.71) vs.(12.13±1.13),P<0.05], pulmonary MDA content in LIPER group was reduced significantly [(0.81±0.05)vs.(1.32±0.09) nmol/mg, P< 0.05], MPO activity in LIPER group was decreased significantly[(2.01±0.17) vs.(4.30±0.22) U/g, P< 0.05], and arterial oxygen partial pressure in LIPER group was significantly increased[(71.0±3.5)vs.(58.5±3.1) mm Hg(1 mm Hg= 0.133 kPa), P<0.05], and SOD activity in LIPER group was significantly increased[(33.78±2.06) vs.(14.21± 1.14) U/mg, P< 0.05].
Conclusion
Limbs ischemic preconditioning can attenuate the lung ischemiareperfusion injury in rats.
Key words:
Lung injury; Lung ischemia; Reperfusion injury; Limbs ischemic perconditioning