Inhibition of rhabdomyosarcoma in nude mice by TNF related apoptosis inducing ligant combined with diamminedichloroplatinum

Objective To observe the effect of inhibition of rhabdomyosareoma in nude mice treated by TNF related apoptosis inducing ligant (TRAIL) combined with diamminedichloroplatinum (DDP), and try to explore the mechanism. Methods Human rhabdomyosareoma cells were inoculated in nude mice subcutaneouly. The nude mice were randomly divided into 4 groups: mice in the TRAIL groups accepted TRAIL injection with the dose of 10μg/Kg; mice in the DDP group accepted DDP in-jection with the dose of 3 mg/kg; mice in the TRAIL + DDP group accepted TRAIL and DDP injection with the abovementioned doses; mice in the control group accepted normal saline injection. The chan-ges of tumor weight and size and the tumor growth inhibition rate were recorded. The functions of liv-er and kidney were evaluated. HE staining was utilized to observe the morphologic changes of livers and kidneys. The expression of Fas protein was detected by flow cytometry (FCM). The expressions of DR4 and DR5 were determined by RT-PCR. Results No significant change of weight was observed in all groups during treatments. Compared with the control group, alanine aminotransferase increased significantly in TRAIL + DDP group and DDP group. No significant change of serum urea nitrogen or creatinine was noted in DDP group and TRAIL + DDP group. The expression of Fas protein of rhab-domyosarcoma cells in TRAIL + DDP group was higher than that in TRAIL group or DDP group. Compared with the control group and TRAIL group, the levels of DR5 and DR4 mRNA were signifi-cantly higher in DDP group and TRAIL + DDP group. The weight of the tumor decreased significantly in 3 treatment groups. Conclusions TRAIL and DDP can significantly inhibit the growth of rhabdomy-osarcoma in nude mice, and the combination of TRAIL and DDP can achieve synergistic effect. Key words: Rhabdomyosarcoma; Tumor necrosis factor receptor-associated peptides and pro-teins;  Cisplatin