Inhibitory effect of resveratrol on proliferation of U87 cells and the action machanisam

Objective To study the inhibitory effect of resveratrol (Res) on proliferation of U87 cells and the possible machanisam.Methods Glioma cell line U87 was treated with different concentrations of Res (0,25,50,100 μmol/L).Cell growth inhibition was detected by using methyl thiazol tetrazolium (MTF) assay.Apotosis was determined using DNA ladder assay and flow cytometry.The molecular events of apoptotic cell death was evaluated by using Western blotting.Results After treatment with 25 μmol/L Res for 24 h,the inhibition rate of U87 cells was 30.96％.Res could inhibit the growth of U87 cell in a-dose and time-dependent manner.There was typical DNA ladder on DNA agarose electrophoresis.Typical subdiploid peak could be detected after treatment with 25 μmol/L Res for 24 h and the apoptosis rate was 11.8％.There was increased protein levels of pro-apoptotic B cell lymphoma/leukemia-2 associated X protein (bax)/bid and decreased protein level of anti-apoptotic B cell lymphoma/leukemia-2 (bcl-2).Conclusion Res can inhibit the growth of U87 cells by inducing typical apoptosis.The downregulation of bcl-2 and up-regulation of bax and bid may be involved in the mechanism. Key words: Resveratrol;  Gliomas;  Apoptosis