Effect and significance of cigarette smoke exposure on the expression of CD80, CD86 by marrow-derived dendritic cells from asthmatics rat

Objective To study the effect of cigarette smoke exposure on the expression of CD80,CD86 and relationship with the activity and immune balance of Iymphocytes by marrow-derived dendritic cells (DCs) from asthmatics rat.Methods Forty Wistar rats were randomly divided into control group,smoke exposure group,asthma group,asthma combined smoke exposure group. Established animal models,then rat bone marrow denved DCs and spleen lymphocytes were cultivated, and mixed lymphocyte responses (MLR) was done.Collected cell and cell supernatant.The expression of CD80 and CD85 was determined by flow cytometric analysis,and the proliferations of lymphocytes was examined with MTT colorimetric assay.The expression of IFN-γ and IL-4 was determined by using the enzyme linked immunosorbent assay (ELISA).Results ① Compared with asthma group,the expression levels of CD86 and CD86 decreased significantly in asthma combined smoke exposure group (P ＜ 0.01,respectively ).Compared with control group,the expression levels of CD80 decreased in asthma combined smoke exposure group significantly ( P ＜0.01).②Compared with control group,the stimulating activity of DCs on allogeneic lymphocytes increased significantly in both asthma group and asthma combined smoke exposure group ( P ＜ 0.01,respectively).Compared with asthma group,the stimulating activity of DCs on allogeneic lymphocytes decreased in asthma combined smoke exposure group significantly ( P ＜0.01).③ Compared with the control group,the expression of IFN-γ decreased and the expression of IL-4 increased remarkably in both the cigarette smoke exposure combined asthma group and the asthma group ( P ＜0.01,respectively).Compared with the asthma group,the expression of IFN-γ decreased and the expression of IL-4 increased remarkably in the cigarette smoke exposure combined asthma group ( P ＜0.01,respectively).④ The expresion levels of CD80 was positively correlated with IFN-γ and the activity of lymphocytes,and negatively correlated with IL-4.The expression levels of CD86 was positively correlated with the activity of lymphocytes.Conclusions Cigarette smoke exposure may inhibit expression of CD80 and CD86 by marrow-derived DCs and reduce DCs stimulating activity on allogeneic lymphocytes in asthmatics rat,which may play an important role in aggravating airway inflammation and immune balance in asthma. Key words: Cigarette smoke;  Bronchial asthma;  Dendritic cells;  Costimulatory molecules;  Immune mechanism