Exploring the autophagy of glioma cells induced by dihydroartemisinin in vitro

Objective To investigate the anti -glioma efficacy of dihydroartemisinin (DHA) and combined with temozolomide (TMZ) on human glioma cell line in vitro.Methods The growth inhibition of DHA on 10 glioma cell lines induced by DHA treatment for 72 h was analyzed by MTT method.TMZ combined with 0.5 μg/ml DHA,and the IC5o value was measured in SKMG -4 cells.The autofluorescent drug monodansylcadaverine (MDC) was used to mark autophagicvacuoles.The autophagy related protein LC3 - B and Beclin - 1,and the apoptosis protein caspase - 3 were analyzed by western blot (WB).Results The IC50 of DHA was different in ten glioma cell lines [ from ( 1.17 ± 0.078 ) μg/ml to (23.568±0.796) μg/ml].In SKMG -4 cells,the autophagicvacuoles were detected in the DHA group,the IC50 of TMZ had significant difference with 0.5 μg/ml DHA contrast to the control group ( P ＜ 0.01 ),and the levels of LC3 - B and Beclin - 1 protein were increased gradually with the increase of DHA concentration,and caspase -3 protein has no difference.Conclusion DHA can inhibit proliferation of glioma cell lines and increase the efficacy of TMZ,and the autophagy may be the mechanism. Key words: Glioma ;  Autophagy ;  Dihydroartemisinin ;  Temozolomide ;