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2010•Journal of ToxicologyOpen access

The effect of DHA compound on Bcl-2 and Bax mRNA transcription in U251 glioma

Sun Ken-ren

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Abstract

Objective To study the anticancer mechanism of DHA compound.Methods The in vitro effect was assessed by cell-culture.RT-PCR was used to investigate the effects of DHA compound on Bcl-2 and Bax mRNA transcription in U251 glioma cell.Results The IC50 of DHA compound on U251 glioma cell was 0.3746 μg/ml(0.3324～0.4168 μg/ml);Compared with control,the content of Bcl-2 mRNA was decreasd significantly and content of Bax mRNA was increased significantly in U251 glioma cell after treated with DHA compound.Conclusion The proliferation of the U251 glioma cell was inhibited by DHA compound.The transcrisption of Bcl-2 was decreased and the transcrisption of Bax mRNA was increased in U251 glioma cell after treated with DHA compound and thus the apoptosis of the cancer cell was promoted.

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Objective To study the anticancer mechanism of DHA compound.Methods The in vitro effect was assessed by cell-culture.RT-PCR was used to investigate the effects of DHA compound on Bcl-2 and Bax mRNA transcription in U251 glioma cell.Results The IC50 of DHA compound on U251 glioma cell was 0.3746 μg/ml(0.3324～0.4168 μg/ml);Compared with control,the content of Bcl-2 mRNA was decreasd significantly and content of Bax mRNA was increased significantly in U251 glioma cell after treated with DHA compound.Conclusion The proliferation of the U251 glioma cell was inhibited by DHA compound.The transcrisption of Bcl-2 was decreased and the transcrisption of Bax mRNA was increased in U251 glioma cell after treated with DHA compound and thus the apoptosis of the cancer cell was promoted.

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Available abstract

Objective To study the anticancer mechanism of DHA compound.Methods The in vitro effect was assessed by cell-culture.RT-PCR was used to investigate the effects of DHA compound on Bcl-2 and Bax mRNA transcription in U251 glioma cell.Results The IC50 of DHA compound on U251 glioma cell was 0.3746 μg/ml(0.3324～0.4168 μg/ml);Compared with control,the content of Bcl-2 mRNA was decreasd significantly and content of Bax mRNA was increased significantly in U251 glioma cell after treated with DHA compound.Conclusion The proliferation of the U251 glioma cell was inhibited by DHA compound.The transcrisption of Bcl-2 was decreased and the transcrisption of Bax mRNA was increased in U251 glioma cell after treated with DHA compound and thus the apoptosis of the cancer cell was promoted.

Key concepts: Glioma, Apoptosis, Messenger RNA, Cell, Chemistry, Transcription (linguistics), Cell culture, In vitro

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The effect of DHA compound on Bcl-2 and Bax mRNA transcription in U251 glioma — Research Paper | ScholarLens