Effects of down-regulation of LRIG1 expression on glioma cells cycle and apoptosis

Objective To investigate the effects of down-regulation of LRIG1 expression on glioma cells cycle and apoptosis and the possible mechanism. Methods Small interfering RNA (siRNA) targeting LRIC1 gene and negative shRNA (neg) were constructed and transfected into CL1S glioma cells. And the cells (siRNA) that stably suppressed LRIG1 expression were selected by G418 (600 mg/L). The change of LRIG1 protein level was measured by Western blotting. The apoptosis rate and cell cycle (0.05 g/L PI, 0.5 g/L RNasine) were analyzed by flow cytometry. Results The LRIG1 protein level in pGene-sil2-LRIGl -shRNA (siRNA) transfected cells was significantly silenced by 54.7%. The propotion of cells in G2/M phase was significantly higher, and apoptosis rate was obviously lower in experimental group than in control group (both P < 0.01). Conclusion Down-regulation of LRIG1 expression can suppress the apoptosis of glioma cells and arrest the cells in G2/M phase. Key words: Glioma;  LRIG1;  Cell cycle